- ￥1350 - 3000
- Proteintech
- 19716-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
PYGM-Specific
- 抗体英文名：
PYGM-Specific Polyclonal antibody
- 抗体名：
PYGM-Specific Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | human skeletal muscle tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19716-1-AP targets PYGM-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphorylase, glycogen, muscle |
| 别名 | PYGM, Myophosphorylase, EC:2.4.1.1 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 97-105 kDa |
| GenBank蛋白编号 | NM_005609 |
| 基因名称 | PYGM |
| Gene ID (NCBI) | 5837 |
| RRID | AB_10642009 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11217 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Nature Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.Authors - Omer Keinan |
| human | WB | Int J Biol Sci PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancerAuthors - Qian Ji |
| mouse | WB | Dis Model Mech Absence of p.R50X Pygm read-through in McArdle disease cellular models.Authors - Guillermo Tarrasó |
| mouse | WB | Cells Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein InteractomeAuthors - Jia-Hua Qu |
| human | WB | Oncol Lett Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.Authors - Lingyu Jiang |
| human | WB | medRxiv Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular DystrophyAuthors - Mustafa Bilal Bayazit |
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