- ￥1350 - 3000
- Proteintech
- 15939-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human FAM38A protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
Piezo1 (extracellular domain)
- 抗体英文名：
Piezo1 (extracellular domain) Polyclonal antibody
- 抗体名：
Piezo1 (extracellular domain) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, SGC-7901 cells, hTERT-RPE1 cells |
| Positive IHC detected in | human skin tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, canine, zebrafish |
| 免疫原 |
CatNo: Ag7791 Product name: Recombinant human FAM38A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-373 aa of BC008073 Sequence: SVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKHHGAVRVHRAGHRQVRARILQRDLALHYVRGAAVRGPHPQALPGHLPGAGDSGAGAGGGVVCQAHLPLPLTGDHDQVDS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 38, member A |
| 别名 | FAM38A, PIEZO1, KIAA0233, Membrane protein induced by beta-amyloid treatment, Piezo-type mechanosensitive ion channel component 1 |
| 计算分子量 | 286 kDa |
| 观测分子量 | 233-286 kDa |
| GenBank蛋白编号 | BC008073 |
| 基因名称 | Piezo1/FAM38A |
| Gene ID (NCBI) | 9780 |
| RRID | AB_2231460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92508 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| zebrafish | IF | Nature Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.Authors - Keat Ying Chan
|
| zebrafish,mouse | WB | Nature Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.Authors - Eisenhoffer George T GT
|
| human | WB | Nature Piezo1 integration of vascular architecture with physiological force.Authors - Jing Li
|
| zebrafish,canine | WB,IF | Nature Mechanical stretch triggers rapid epithelial cell division through Piezo1.Authors - S A Gudipaty
|
| mouse | IHC | Cell RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.Authors - Erika Sugisawa |
| human | WB | Blood Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.Authors - ShengPeng Wang
|
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
production. Following an 8 hour incubation, the filters are marked, removed, washed, blocked and incubated with the AP-fusion protein. Then, the filters are washed again and AP substrate (NBT/BCIP) is added indicating the positive plaques. These positive
膜蛋白超家族的研究。目前，这一大家族含有28个受体和18个配体。TNF受体超家族的成员都含有富含胱氨酸的胞外结构域(cysteine rich extracellular domain)。基于受体胞内部分不同，TNF超家族又分为两大类：死亡受体家族和非死亡受体家族。肿瘤坏死因子超家族在免疫与炎症反应中有重要作用，以下着重介绍与细胞凋亡关系密切的死亡受体家族。 (一)死亡受体家族(DeathReceptorFamily) 该家族的胞内部分都含有约80个氨基酸残基组成的介导凋亡区域，即死亡
结构部分由 E1 和 E2 两个亚结构域组成，胞质内区域称为 E3 结构域 ［2］。 图片来源于：Coburger I et al．Analysis of the overall structure of the multi-domain amyloid precursor protein (APP) Aβ是由 APP 编码的蛋白经一系列水解酶酶切产生（学霸自行跳过文字，看图脑补即可）。APP 在跨膜分泌过程中共有 2 条水解途径，即不产生 AD 的非淀粉
技术资料暂无技术资料 索取技术资料
