- ￥1350 - 3000
- Proteintech
- 14999-1-AP
- 2025年10月13日
- WB, IHC, FC (Intra), IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TXN protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), IP, ELISA
- 靶点：
Thioredoxin
- 抗体英文名：
Thioredoxin Polyclonal antibody
- 抗体名：
Thioredoxin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, K562 cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14999-1-AP targets Thioredoxin in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, chicken |
| 免疫原 |
CatNo: Ag6989 Product name: Recombinant human TXN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-105 aa of BC003377 Sequence: MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin |
| 别名 | TXN, TRDX, THIO, Surface-associated sulphydryl protein, Hom s Trx |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC003377 |
| 基因名称 | TXN |
| Gene ID (NCBI) | 7295 |
| RRID | AB_2272597 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10599 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.Authors - Lingbing Zhang |
| mouse | WB | Acta Pharm Sin B Fluorine-thiol displacement probes for acetaminophen's hepatotoxicityAuthors - Benjamin L Prather |
| human | WB | Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.Authors - Ziwen Wang |
Nano Converg Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle.Authors - Myeong-Jun Lee | ||
| human | WB | Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neuronsAuthors - Carissa L Sirois |
| mouse | WB | Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.Authors - Wu Hongtan H |
Thioredoxin and Redox Regulation of the Nuclear Receptor
Oxidative stress evokes various cellular responses including alteration of gene expression to preserve cellular homeostasis (1 ,2 ). Thioredoxin (TRX) is a small ubiquitous protein with protein thiol-reducing activity and has been shown
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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