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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Galectin 1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, ELISA
- 靶点:
Galectin-1
- 抗体英文名:
Galectin-1 Polyclonal antibody
- 抗体名:
Galectin-1 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, HCT 116 cells, MCF-7 cells, U2OS cells, NIH/3T3 cells, PC-12 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11858-1-AP targets Galectin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lectin, galactoside-binding, soluble, 1 |
| 别名 | Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1, Galaptin |
| 计算分子量 | 15 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC020675 |
| 基因名称 | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_2234673 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09382 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,human | WB,IF | Dev Cell Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorptionAuthors - Mobai Li |
| human | IF | Front Med Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysisAuthors - Weiwei Xian |
| human | WB | Cell Rep Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.Authors - Krishna S Tummala |
| human | WB | Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.Authors - Chen Yang |
| human | WB,IHC | Front Oncol Prognostic Value and Biological Function of Galectins in Malignant Glioma.Authors - Hongtao Zhu |
| mouse | IHC | Biomed Pharmacother Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.Authors - Yoojin Seo |
: CSB-BP619964 HU >> Baculovirus CSB-MP619964 HU >> Mammalian cell PD1 多克隆抗体 Rabbit anti PD1 货号:CSB-PA619964LA01 HU 识别种属:human,mouse 应用检测:WB、IHC、IF 等 TIM3 重组蛋白 货号:CSB-EP010145 HU TIM3 多克隆抗体
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实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
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