- ￥1350 - 3000
- Proteintech
- 11256-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Annexin A2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
Annexin A2
- 抗体英文名：
Annexin A2 Polyclonal antibody
- 抗体名：
Annexin A2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse placenta tissue, THP-1 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse placenta tissue, human bowen disease tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
| Positive FC (Intra) detected in | K-562 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, monkey, chicken, zebrafish, bovine |
| 免疫原 |
CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | annexin A2 |
| 别名 | ANXA2, Annexin 2, Annexin II, Annexin-2, ANX2 |
| 计算分子量 | 339 aa, 39 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC016774 |
| 基因名称 | Annexin A2 |
| Gene ID (NCBI) | 302 |
| ENSEMBL Gene ID | ENSG00000182718 |
| RRID | AB_2057311 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07355 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.Authors - Di Huang | |
| mouse | WB | Cell Mol Immunol Malonate promotes CD8+ T cell memory formation via protein malonylationAuthors - Qianqian Duan |
| mouse | WB | Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular VesiclesAuthors - Pei Jiang |
| human | WB | Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.Authors - Xiaochao Tan |
| human | WB | Nat Chem Biol YAP-dependent proliferation by a small molecule targeting annexin A2.Authors - Sophia Z Shalhout
|
| human | WB | Nat Commun PMID: 31792210Authors - Sunwang Xu |
。根据Ap峰的百分率可检测凋亡细胞数。在本组研究中PI法凋亡检出率为27.19%,显著低于TUNEL法及Annexin V/PI法。其检出率低的原因主要是凋亡早期尽管有DNA 裂点形成，但尚未出现DNA 片段的大量丢失，因此该法不能检出早期凋亡细胞。次要原因是发生于S期或G2 /M期的凋亡细胞，即使有DNA 含量降低，其实际含量也不低于二倍体细胞所含的DNA 。国内李莉报道采用与本文同一动物模型PI漏检率高达42.8%。此外PI法还存在错检，一部分坏死细胞碎片发生低荧光，位于二倍体峰前，造成PI法错检
打印机打印结果。 2 结果 2.1 PI检测法 在正常二倍体细胞DNA峰(G1峰)前出现一亚二倍体凋亡峰,称Ap峰(图1)。 2.2 TUNEL检测法 根据FITC-dUTP掺入量的多少区分正常及凋亡细胞(图2)。 2.3 FITC-Annexin V/PI检测法 正常细胞FITC及PI均低染(FITC- PI- )，在流式细胞分析图上为R1 区细胞簇。凋亡细胞FITC高染而PI低染(FITC+ PI- )，图上显示为R2 区细胞簇。死亡细胞FITC及PI均
；加入羊抗人α2M多克隆抗体，37~C 2h，洗涤；加入HRP标记的兔抗羊IsC，孵育后加入底物呈色。终止反应后在酶标仪上读取492nm波长的吸光度值。按常规绘制标准曲线，算出待测血a2M含量。 3．正常值范围与临床意义 免疫扩散法测得正常男性血清a2M为1．5～3．5z／L，女性为1．35～4．2g／L。2—4岁儿童血清a2M约为成人的2～3倍。更年期妇女血清e2M含量也较高。ELBA法检测正常人尿液中Q，M含量均低于0．026mg／L
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