- ￥1350 - 3000
- Proteintech
- 10934-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CCND2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
Cyclin D2
- 抗体英文名：
Cyclin D2 Polyclonal antibody
- 抗体名：
Cyclin D2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Caco-2 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, HEK-293 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10934-1-AP targets Cyclin D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, bovine, goat |
| 免疫原 |
CatNo: Ag1377 Product name: Recombinant human CCND2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-289 aa of BC010958 Sequence: MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQLLGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWNLAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQIEAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin D2 |
| 别名 | CCND2, G1/S-specific cyclin-D2, G1/S specific cyclin D2, Cyclin D |
| 计算分子量 | 32 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC010958 |
| 基因名称 | Cyclin D2 |
| Gene ID (NCBI) | 894 |
| RRID | AB_2275319 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| mouse | Nature SOX2 controls tumour initiation and cancer stem-cell functions in squamous-cell carcinoma.Authors - Soufiane Boumahdi | |
| human | WB | Cancer Cell The Oncogenic Transcription Factor RUNX1/ETO Corrupts Cell Cycle Regulation to Drive Leukemic Transformation.Authors - Natalia Martinez-Soria
|
| human | WB | Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.Authors - Xi Jin |
| mouse | WB | Dev Cell Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.Authors - Yan Gong |
| mouse | Kidney Int Transcription factor MafB may play an important role in secondary hyperparathyroidism.Authors - Naoki Morito | |
| human | IHC | Front Immunol Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancerAuthors - Qianshan Hou |
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
Immunodetection of cyclin D1 and D2 /D3 using 488/630 nm dual laser flow cytometry William Telford Hospital for Special Surgery
技术资料暂无技术资料 索取技术资料
