- ￥1350 - 3000
- Proteintech
- 10928-1-AP
- 2025年10月13日
- WB, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HSD11B1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
HSD11B1
- 抗体英文名：
HSD11B1 Polyclonal antibody
- 抗体名：
HSD11B1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | L02 cells, mouse liver tissue, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10928-1-AP targets HSD11B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1361 Product name: Recombinant human HSD11B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-292 aa of BC012593 Sequence: MAFMKKYLLPILGLFMAYYYYSANEEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETLQKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHITNTSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSLAGKVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTETAMKAVSGIVHMQAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNPCRKILEFLYSTSYNMDRFINK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid (11-beta) dehydrogenase 1 |
| 别名 | 7-oxosteroid reductase, 11-DH, 11-beta-hydroxysteroid dehydrogenase 1, 11-beta-HSD1, 11 DH |
| 计算分子量 | 36 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC012593 |
| 基因名称 | HSD11B1 |
| Gene ID (NCBI) | 3290 |
| RRID | AB_2119527 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28845 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confirming the dimeric nature of 11-beta-HSD1 in liver. In omental adipose tissue, an additional band of 50 kDa was strongly expressed(PMID:16914598).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Brain Behav Immun HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure.Authors - Bin Xu |
| human | WB | J Assist Reprod Genet Effect of luteinizing hormone concentration on transcriptome and subcellular organelle phenotype of ovarian granulosa cells.Authors - Yu-Ting Wan |
| mouse | IF,WB,IHC | Int J Biol Sci 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo SignalingAuthors - Hanwen Li |
| human | WB | Gynecol Endocrinol N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathwayAuthors - Yuan-Xue Jing |
| mouse | WB | Geroscience Adipose chemokine ligand CX3CL1 contributes to maintaining the hippocampal BDNF level, and the effect is attenuated in advanced ageAuthors - Yoshinori Takei
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因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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