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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human LAMA4 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
LAMA4 (Isoform 3)
- 抗体英文名:
LAMA4 (Isoform 3) Polyclonal antibody
- 抗体名:
LAMA4 (Isoform 3) Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | human heart tissue, human skeletal muscle tissue |
| Positive IHC detected in | human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0736 Product name: Recombinant human LAMA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-120 aa of BC004241 Sequence: MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGRQDPPETSEPRVALGRLPPAAEVQCPCHCHPAGAPAPPRAVPHSSFSLSPPLSSPQCLESFTWARSVRKLEIKSFPL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | laminin, alpha 4 |
| 别名 | LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4 |
| 计算分子量 | 13 kDa |
| 观测分子量 | 11-13 kDa |
| GenBank蛋白编号 | BC004241 |
| 基因名称 | Laminin alpha 4/LAMA4 |
| Gene ID (NCBI) | 3910 |
| RRID | AB_2234461 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16363 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Arthritis Rheum PMID: 21337318Authors - Gutierrez-Roelens I I |
| human | IHC | Sci Rep Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.Authors - Katsutaka Mitachi |
| mouse | IHC | Am J Physiol Heart Circ Physiol Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.Authors - Li Lin |
| human | IHC | Neuromuscul Disord Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.Authors - Doppler K K |
| human | IHC | J Neurosci Res Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.Authors - Xu Hongmin H |
| human | WB,IHC | Cancer Cell Int MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.Authors - Zhi-Xue Yang |
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
发生,最好在特异性抗体处理切片之前,选择与二抗种属相同的非免疫血清封闭电荷,阻止一抗与之结合,抑制非特异性背景着色。常用方法是用2%~10%羊血清或2%~5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固,所以最好不要冲洗,倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7.第一抗体孵育 滴加特异性一抗于切片上,4℃孵育24~48小时,或室温孵育过夜,也可在37~C孵育1~2小时。之后,用PBS冲洗3次,每次5分钟。一抗分为多克隆抗体和单克
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