- ￥1350 - 3000
- Proteintech
- 10087-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Annexin IV protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
Annexin A4
- 抗体英文名：
Annexin A4 Polyclonal antibody
- 抗体名：
Annexin A4 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HuH-7 cells, RAW 264.7 cells, C6 |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10087-1-AP targets Annexin A4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0130 Product name: Recombinant human Annexin IV protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-202 aa of BC000182 Sequence: GTDEDAIISVLAYRNTAQRQEIRTAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVLVSLSAGGRDEGNYLDDALVRQDAQDLYEAGEKKWGTDEVKFLTVLCSR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | annexin A4 |
| 别名 | ANXA4, ANX4, Annexin-4, Annexin IV, Annexin 4 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC000182 |
| 基因名称 | Annexin A4 |
| Gene ID (NCBI) | 307 |
| RRID | AB_2057474 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09525 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Mol Cell Proteomics Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.Authors - Rogers Ronan S RS |
| human | WB | Aging (Albany NY) Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.Authors - Ya Peng |
| mouse | WB | J Immunol Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.Authors - Kulik Liudmila L |
| human | WB | Mol Cell Proteomics Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.Authors - Hussein Kaddour |
| human | WB,IHC | Sci Rep Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.Authors - Kewu Jiang |
| human | WB | PLoS One Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.Authors - Qi Ying |
文章：推测损坏神经元的 Aβ在大脑中进行着秘密活动——保护大脑免于病原体的侵袭。研究者发现 Aβ能够以「抗菌肽」的身份防御微生物感染；而且，对于一些微生物而言，它的抗菌效果超出青霉素 100 倍！因此，Aβ或许不需要完全清除。 人类越来越强大，面对最后一大块未被征服的科学领域——脑，也在想方设法的探索它的「私生活」。在探索的道路上，华美已经为您准备好了 APP 的重组蛋白、多克隆抗体和单克隆抗体。 淀粉样肽 beta A4 重组蛋白 货号：CSB-RP
【专题讨论】Western blot一抗的选择：单抗or多抗？参与讨论赢取10个丁当！
；millipore旗下的chemicon和upstate也非常好。我觉得多克隆抗体更常见更实用。 链接：http://www.dxy.cn/bbs/topic/14721019?keywords=western%20blot%20%E5%A4%9A%E6%8A%97 @yihui88117 ，认为：一般是单抗好于多抗！很多公司都有抗体买！国外的抗体要比国内的抗体好一点！但是象乙肝E抗体我觉的没有必要买国外的，因为太贵了！ 链接：http://www.dxy.cn/bbs/topic/1626604
。根据Ap峰的百分率可检测凋亡细胞数。在本组研究中PI法凋亡检出率为27.19%,显著低于TUNEL法及Annexin V/PI法。其检出率低的原因主要是凋亡早期尽管有DNA 裂点形成，但尚未出现DNA 片段的大量丢失，因此该法不能检出早期凋亡细胞。次要原因是发生于S期或G2 /M期的凋亡细胞，即使有DNA 含量降低，其实际含量也不低于二倍体细胞所含的DNA 。国内李莉报道采用与本文同一动物模型PI漏检率高达42.8%。此外PI法还存在错检，一部分坏死细胞碎片发生低荧光，位于二倍体峰前，造成PI法错检
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