- ￥1350 - 3000
- Proteintech
- 10380-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Caspase 9 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
Caspase 9/P35
- 抗体英文名：
Caspase 9/P35 Polyclonal antibody
- 抗体名：
Caspase 9/P35 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, Staurosporine treated Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse lung tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10380-1-AP targets Caspase 9/P35 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, RIP, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep, goat |
| 免疫原 |
CatNo: Ag0404 Product name: Recombinant human Caspase 9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 138-389 aa of BC002452 Sequence: DVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVAN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 9, apoptosis-related cysteine peptidase |
| 别名 | CASP9, Caspase 9, Caspase 9/p35/p10, APAF 3, APAF3 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa, 30-39 kDa |
| GenBank蛋白编号 | BC002452 |
| 基因名称 | Caspase 9 |
| Gene ID (NCBI) | 842 |
| RRID | AB_2068632 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55211 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
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|---|---|---|
| human | WB | Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinomaAuthors - Lei Xu |
| mouse | WB,IHC | Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissectionAuthors - Yumei Que |
| rat | WB | Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse modelAuthors - Mengyan Hu |
| human | WB | Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.Authors - Xiaowei Zhang |
| human | WB | Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancerAuthors - Shaofeng Duan |
| rat | WB | Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.Authors - Zhi Zheng |
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase,PARP]等的裂解。方法：收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
的Caspase-3及对底物多聚（ADP-核糖）聚合酶[poly（ADP-ribose）polymerase，PARP]等的裂解。方法： 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T（含0.05% Tween 20的TBS）洗3次，5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
一 caspase家族蛋白酶的组成 未活化的caspase家族蛋白酶是以酶原形式存在的，酶原的氨基端有一段被称为“原结构域”(pro-domain)的序列。酶原活化时不但要将原结构域切除，并且要将剩余部分剪切成一大一小两个亚基，分别称为P20和P10，活性酶就是由这两种亚基以(P20/P10)2的形式组成的。这种活化反应也是Asp特异的，剪切发生在酶原中保守序列的Asp与其后的氨基酸残基
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