ACL-4 medium (serum-free)The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:0.02 mg/ml insulin0.01 mg/ml transferrin25 nM sodium selenite (final conc.)50 nM Hydrocortisone (final conc.)1 ng/ml Epidermal Growth Factor (do not filter)0.01 mM ethanolamine (final conc.)0.01 mM phosphorylethanolamine (final conc.)100 pM triiodothyronine (final conc.)0.5% (w/v) bovine serum albumin (final conc.)0.5 mM sodium pyruvate (final conc.)extra 2mM L-glutamine (for final conc. of 4.5mM)