产品名称:Klenow片段
英文名称:Klenow Fragment
产品货号:GS2043
产品规格:200U
储存条件:冷冻(-20℃)
概述
Klenow Fragment is the large fragment of DNA polymerase I.It exhibits 5→3 polymerase activity and 3→5 exonuclease(proofreading)activity,but lacks 5→3 exonuclease activity of DNA polymerase I.
特点
Incorporates modified nucleotides(e.g.,Cy3-,Cy5-,aminoallyl-,biotin-,digoxigenin- and fluorescently-labeled nucleotides)Active in restriction enzyme,PCR,RT,and T4 DNA Ligase buffers
应用
DNA blunting by fill-in 5-overhangs Random-primed DNA labeling Labeling by fill-in 5-overhangs of dsDNA
技术规格
Unit definition
Unit One unit of the enzyme catalyzes the incorporation of 10nmolof deoxyribonucleotides into a polynucleotide fraction(adsorbed on DE-81)in 30minutesat 37℃,using poly(dA-dT)·poly(dA-dT)as a template·primer.
来源
E.coli cells with a cloned fragment of the polA gene