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DOB-Glucose w/ 2% Raffinose Powder, 250 grams(Sunrise Science; CAT# 1654-250)
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Sunrise Science
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文献和实验0.1 g/L葡萄糖 glucose 14g/L YNB Yeast Nitrogen Base with ammonium sulfate w/out amino acids (BD Difco 233520) 5.4 g/L Na2HPO4 7.4 g/L NaH2PO4 YEPGR or YPGR = Yeast Extract Peptone Galactose/Raffinose 10g 酵母粉yeast extract
(15:60:25) Combine 150 ml of glacial acetic acid, 600 ml of nbutanol and 250 ml of water. Aceto-orcein Add 2.0 grams of orcein to 45 ml of glacial acetic acid. Bring to a boil and continue to heat
To prepare cultures, add required amount of medium to a clean petri dish (15 ml in a 100 x 15 mm Petri dish for 500 mg cultures or 7.5 ml in a 60 x 15 mm Petri dish for 250 mg cultures). Place the dish on a balance, tare, and add tissue explants until desired
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