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DOB Powder, 1 kg(Sunrise Science; CAT# 1650-1KG)
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
FROM CEREAL LEAVES 1)extract nuclei in nuclear homogenization buffer NHB from 1kg leaves (see nuclei prep steps 1-8) 2)resuspend in 35ml TE 3)add 35ml 2x nuclear lysis buffer NLB (see nucext.rcp) 4)add proteainase K to 0.2mg/ml 5)incubate 37
Twelve catheter connection assemblies with metal spring covering (PlasticsOne, cat. no. C313CS) Scale with 1 g accuracy and at least 500‐g capacity for weighting rats
4 litres $104 ("Laboratory grade") Sigma 3 kg $42 (no purity details) Acros Organics (seems to be part of Fisher) sell three grades: 2.5 litres $24 (99%) 1 kg $23 (GC) 500 ml $36 (scintillation grade) If the 99% stuff is OK for histology
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