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- 英文名:
DOBA Powder, 500 grams(Sunrise Science; CAT# 1651-500)
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Sunrise Science
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文献和实验Purpose Materials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 107718 ml cold H2 O2 ml 10x PBSM199 for HUVECs: 1L powder pocket
Rhodamine-Phalloidin/Calcofluor Staining
size coverslip and waiting for capilary action to draw the liquid out thus flattening the cells. Reagents Formaldehyde Polysciences, 10% EM Grade Cat #04018 10XPBS 80 grams NaCl + 2 grams KCl + 14.4 grams Na2HPO
Preparation of bOG-DOPG mixed micelles
Materials: All glassware must be acid washed, rinsed thoroughly with water then rinsed with acetone and dried. Redistill acetone. Recrystallizing BOG: 1) Add 5 grams of Octyl-b-D-glucopyranoside (bOG = Calbiochem #494459
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