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- 351186
- 美国
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
BD
- 库存：
大量
- 现货状态：
询
- 保修期：
询
- 规格：
5
主要经营的试剂品牌：
- Roche试剂，部分常用现货，周三定货，2-3周到货。
- GE试剂，耗材，部分常用现货，周二，周五定货，3周左右到货。
- R&D试剂，周三定货，2-3周到货。
- SIGMA试剂，周一，五定货，3周左右到货。
- Millipore试剂，耗材，每周三定货，2-3周到货。
- Invitrogen试剂，部分现货，周三定货，2-3周到货。
- ABCAM试剂，周三定货，2-3周到货。
- eBioscience试剂，部分常用现货，周三定货，2-3周到货。
- STANT CRUZ试剂，周三定货，2-3周到货。
- Biolegend试剂，周三定货，2-3周到货。
- Peprotech试剂，部分常用现货，周三定货，2-3周到货。
- 罗氏原料，请来电来邮咨询。
BD耗材
| 351186 | PLATEFEEDERTRAYW/LIDFOR24WINSERTS | 5 |
| 351190 | PLATELIBRARY96WELLPPROUNDWELL | 100 |
| 352001 | TUBE14ML17X100MMPSW/SNCAP1/PAC 1000/cs | 500 |
| 352002 | TUBE5ML12X75MMPPNOCAPNS1000/PAC | 1000 |
| 352003 | TUBE5ML12X75MMSTYLEPSW/SNCAP1/PAC500/cs | 500 |
| 352006 | TUBE14ML17X100MMPPW/SN CAP1/PAC 1000/cs | 500 |
| 352008 | TUBE5ML12X75MMSTYLEPSNS1000/PAC | 1000 |
| 352017 | TUBE14ML17X100MMPSNO CAP125/PAC | 1000 |
| 352018 | TUBE14ML17X100MMPPNOCAP125/PAC1000/cs | 1000 |
| 352025 | TUBE16ML16X125MMPSW/SCCAP125/PAC1000/cs | 1000 |
| 352027 | TUBE8ML13X100MMPSW/SCCAP125/PAC | 1000 |
| 352032 | CAPTUBE 5ML1275MMSTYLEPETG500P2000CS | 2000 |
| 352037 | TUBE16ML16X125MMPSW/SCCAP1/PAC500/cs | 500 |
| 352045 | TUBE19ML16X150MMPSW/SCCAP1/PAC | 500 |
| 352051 | TUBE14ML17X100MMPSW/SN CAP125/PAC 1000/cs | 1000 |
| 352052 | TUBE5ML12X75MMPSW/OCAP125/PAC | 1000 |
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文献和实验Preparation of Feeder Layers And SNL Stocks
incubator, labelling the tray with the clone number (e.g., SNL 76/7), the passage number, and the date. NOTE: The 15 cm plates will be used upon confluence to prepare the feeder plates. The one 10 cm plate will be used
OUTLINE OF PROCEEDINGS USED IN TISSUE CULTURE TRANSFECTIONS
. Mix evenly and incubate for 24 hours. 3. Split the cells to 1.5 x 105 cells per 100mm culture dish. Use I dish per transfection and one for the negative control. Remember to freeze any leftover cells. Mix evenly and incubate for 24 hours.
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
V 4. ASAP using a pasteur pipette transfer cells from curvette A to a 10 ml tube containing 4 mls medium, then plate 5 x 1 ml cells onto five 10cm dia petri dishes containing NeoPMEF's and 10 ml medium. Label plates 4-8. B to a 10 ml tube containing 1ml
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