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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
KLH | Keyhole limpet hemocyanin, HRP-conjugated (40 µg)
- 规格:
40ug
| Immunogen |
| Purified keyhole limpet hemocyanin (KLH), whole molecule, |
|
Host Rabbit
|
|
Clonality Polyclonal
|
|
Purity Affinity purified serum in PBS, pH 7.4
|
|
Format Liquid
|
|
Quantity 40 µg
|
|
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
|
|
Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
|
|
Recommended dilution
|
| 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL) |
|
Expected | apparent MW
|
| ca. 400 kDa/subunit |
名称:KLH | Keyhole limpet hemocyanin, HRP-conjugated (40 µg)
货号:AS99 001-HRP
品牌:Agrisera
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文献和实验The T-Dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents
production) to an introduced antigen (i.e., keyhole limpet hemocyanin (KLH)) in rats or mice treated with a chemical (e.g., a known immunotoxicant and/or a new drug candidate). The TDAR model of competent immune function requires the participation of multiple
Preparation Of Peptide-KLH Conjugates For Immunization
will not couple well. You may need to regenerate the thiol groups by reducing the peptide with excess DTT and running a P2 column.2. Coupling of Peptide to KLHThis recipe is for two bunnies for about five injections per bunny(1) Weigh out 100mg of keyhole limpet
How Do I make an Antibody against a Peptides
-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids (glutamic, aspartic acid or 5'-phosphate) to the carrier protein. Keyhole limpet
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