- ￥4214
- Agrisera
- 瑞典
- AS17 4114
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Gamma CA | Gamma Carbonic anhydrases
- 规格：
50ul
| Immunogen |
| KLH-conjugated peptide derived from Arabidopsis thaliana Gamma-Carbonic anhydrase, UniProt: Q9C6B3, TAIR: AT1G47260 |
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Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 50 µl
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Reconstitution For reconstitution add 50 µl of sterile water. Serum contains 0.1 % sodium azide as preservative.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 1000 (WB)
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Expected | apparent MW 9.97 KDa (gamma-CA1), 30KDa (gamma-CA2) strongest band, 27.83 (gamma-CA3), 25.08 KDa Arabidopsis thaliana proteins
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名称：Gamma CA | Gamma Carbonic anhydrases
货号：AS17 4114
品牌：Agrisera
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文献和实验Irradiating Cells - Once cells are confluent tighten the lid of the flasks and take up to the gamma source in the animal house on the third floor (PMCI), if you have a number of flasks you can trypsinise all the flasks and place
containing 10ml of PMEF media. Incubate at 37oC for two days or until confluent IRRADIATING CELLS Once cells are confluent tighten the lid of the flask and take up to the gamma source in the animal house on the third floor (PMCI) Irradiate
FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
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