p70S6K|Ribosomal protein S6 ki

Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification

ribosomal S6 kinase (RSK) and p70 S6 kinase (S6K) that are present in the NFAT:DNA complex. We further demonstrated that RSK and S6K binds to and physically interacts with NFATc4. Similar oligonucleotide affinity-binding approach can be coupled

Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay

Immunoprecipitation is one of the most commonly used techniques to study protein-protein interaction in vivo. There are three major steps involved in an immunoprecipitation procedure: 1) lysis of the cells to make the antigen

Measurement of Phosphoinositide 3-Kinase Activity

only PtdIns as a substrate (e.g., mammalian PtdIns 3-kinase and yeast Vps34p) ( 1 ). Generally, PI 3 kinases are now regarded as an important intracellular signal upstream of a variety of biochemical (e.g., activation of Akt/protein kinase B and/or p70