- ￥4214
- Agrisera
- 瑞典
- AS16 3951
- 2026年02月24日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
HRP | Horseradish peroxidase
- 规格：
50ul
| Immunogen |
| Native peroxidase isolated and purified from horseradish. |
|
Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 50 µl
|
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Reconstitution For reconstitution add 50 µl of sterile destilled water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications ELISA (ELISA), Western blot (WB)
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Recommended dilution 1 : 1000-1 : 5000 (WB)
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Expected | apparent MW
|
| 38.6 kDa |
名称：HRP | Horseradish peroxidase
货号：AS16 3951
品牌：Agrisera
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文献和实验Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups
底物范围较广，包括二氨基联苯胺 (DAB)、氯萘酚和氨乙基咔唑等。 二氨基联苯胺/金属盐 DAB 是辣根过氧化物酶最敏感、常用的底物。它产生强烈的棕色产物，在水和醇中不溶解。多数情况下，推荐在 DAB 中加入不同的金属离子。当加入金属盐如钴、镍至底物液中，辣根过氧化物酶可以更加敏感。此类反应产物呈暗蓝灰色至黑色，且在水及醇中稳定。DAB/金属盐染色应用的组织染色范围较广。 1. 需要的溶液和特殊设备 DAB，0.05 mol/L Tris 缓冲液 (pH7.6), 0.3％ (W
Preparation of Horseradish Peroxidase-Labeled Probes
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) was first described by Renz at EMBL in 1984 (1 ). The methodology was combined with enhanced chemiluminescence (2 ) (a light producing HRP catalyzed reaction
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