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Frozen Vial (1x106 Cells )
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文献和实验Isolation of Primary Fibroblasts from Mouse Embryos
the plates @ 37o C until the cells have reached confluence (approx. 5 days). (The primary fibroblasts will be the only cells that attach and proliferate.) 9. At confluence, split the plate 1:3 by doing the following: a. Wash the plate twice with PBS. b. Add
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
You will need: 13.5 day pregnant mouse (we use MTK NEO inbred white mice) 2 sets sterile instruments one containing a pair of curved forceps and a pair of iris scissors one containing two pairs curved forceps, one pair iris
Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
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