- $615
- LSBio
- To be determined
- LS-F2228
- 美国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
Human PBK / TOPK ELISA Kit (Cell-Based ELISA) - LS-F2228
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Product Description
LS-F2228 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human PBK / TOPK in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
PBK / TOPK
Reactivity
Human
Specificity
PBK/TOPK
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
- Seed cells into wells and incubate overnight at 37°C, 5% CO2.
- Apply desired treatment conditions.
- Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
- Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
- Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
- Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
- Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About PBK / TOPK
Q96KB5 NM_018492 NP_060962.2
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Cell-Based ELISA Platform Overview
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有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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