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- 11131D
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
- 供应商：
研卉生物
- 规格：
5 x 2 mL
描述
Dynabeads® Human T-Activator CD3/CD28 用于活化和扩增人 T 细胞和 T 细胞克隆，以作研究之用。Dynabeads® CD3/CD28 T Cell Expander 提供了活化和扩增 T 细胞的简单方法，无需抗原呈递细胞或抗原。 Dynabeads® Human T-Activator CD3/CD28 是均匀的 4.5 µm 超顺磁珠，与抗 CD3 和抗 CD28 单克隆抗体的优化混合物偶联。 通过使抗 CD3 和抗 CD28 抗体与 Dynabeads® 结合，这种微珠可以提供 T 细胞活化和扩增所需的原始和共刺激信号。
起始样品
来自全血或血沉棕黄层的 MNC/PBMC，或者包含 CD3+ T 细胞、CD4+ 或 CD8+ T 细胞、调节性 T 细胞或 T 细胞克隆的 T 细胞亚群。
规格
| Cell Type: | T Cells (Whole Population) |
|---|---|
| Clonality: | Monoclonal |
| Final Product: | Cells |
| Product Line: | DYNAL®, Dynabeads® |
| Product Size: | 2 mL |
| Reactivity: | Human |
| Sample Type (Specific): | Cell Cultures, PBMC |
| Target Species: | Human |
| Shipping Condition: | Room Temperature |
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文献和实验Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
containing 30 U/ml rIL-2. 4. Re-Stimulation Cell cultures showing signs of exhaustion (typically at day 7-10 of expansion) can be re-stimulated several times by adding fresh Dynabeads Mouse T-Activator CD3/CD28 and rIL-2. The CD8 T
Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
Collection, Separation, and Activation of Human T Lymphocytes
) (5 ,6 ). CD28 ligation provides cyclosporin A-resistant biochemical signals to T cells, which are an absolute requirement to drive proliferation and IL-2 production from CD3-stimulated T cells, as well as enhance cell survival (5 ,6 ). In contrast, CTLA
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