重组人神经生长因子前体 hproNGF

The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects.ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.

·Description
Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25kDa.

·Quality Control
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.
Endotoxin: Less than 0.1ng/μg (1.0EU/μg) of rHuProNGF as determined by LAL test.

·Formulation
Sterile and Lyophilized aliquots with a 0.2um filtered protein solution containing 20mM PBS pH7.4.

·Reconstitution
It is recommended to reconstitute the lyophilized rHuProNGF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other solutions.

·Storage
Lyophilized rHuProNGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C, which is stable for up to twelve months from date of receipt. Upon reconstitution rHuProNGF should be stored at 4°C between 2-7 days and aliquots of reconstituted samples with 0.1% HSA or BSA are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.