LS-F55210 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse PARK7 / DJ-1 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of PARK7 / DJ-1 as low as 0.059 nanograms per millilter.
This assay has high sensitivity and excellent specificity for detection of Parkinson Disease Protein 7 (PARK7). No significant cross-reactivity or interference between Parkinson Disease Protein 7 (PARK7) and analogues was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.059 ng/ml
Assay Time
3 h
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Protocol
Prepare all reagents, samples and standards.
Add 100 uL standard or sample to each well.
Incubate 2 hours at 37ºC.
Aspirate and add 100 uL prepared Detection Reagent A.