Anti-CRYAB Polyclonal Antibody

产品简介

单位	支
英文名称	Anti-CRYAB Polyclonal Antibody
别名	CRYA2;CTPP2;HSPB5;CMD1II
宿主	Rabbit
稀释比例	WB 1:500-2000. IHC 1:20-100.
Gene ID	1410
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P02511
蛋白分子量	20kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm
免疫原	Recombinant protein of human CRYAB
亚型	IgG
性状	液体
纯化方法	Affinity purification
背景资料	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Public Immunogen Range	Recombinant protein of human CRYAB
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with CRYAB antibody diluted at 1:1000; Lane :Moue heart,Mouse skeletal muscle ,Rat heart,Rat skeletal muscle

Immunohistochemistry of paraffin-embedded Human thyroid cancer with CRYAB antibody diluted at 1:50