Anti-B4GALT1 Polyclonal Antibo

产品简介

单位	支
英文名称	Anti-B4GALT1 Polyclonal Antibody
别名	B4GAL-T1;CDG2D;GGTB2;GT1;GTB;beta4Gal-T1
宿主	Rabbit
稀释比例	WB 1:500-2000.
Gene ID	2683
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P15291
蛋白分子量	44kDa
储存液	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
亚细胞定位	Cell membrane Cell projection Cell surface Golgi apparatus Golgi stack membrane Secreted Single-pass type II membrane protein filopodium
免疫原	Recombinant protein of human B4GALT1
亚型	IgG
性状	液体
纯化方法	Affinity purification
背景资料	This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose < > UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
Public Immunogen Range	Recombinant protein of human B4GALT1
克隆类型	Polyclonal Antibody
交叉反应	Human
应用	WB
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with B4GALT1 antibody diluted at 1:1000