- ￥960 - 1600
- Solarbio
- 北京
- K003344P
- 2025年10月16日
- Rabbit
- Human Mouse Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
- 亚型：
IgG
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human Mouse Rat
- 目录编号：
K003344P
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 靶点：
Symmetric DiMethyl-Histone H3-R2
- 抗体英文名：
Anti-Symmetric DiMethyl-Histone H3-R2 Polyclonal Antibody
- 抗体名：
H3.4;H3/g;H3FT;H3t
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
产品简介
备注： 以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。 These protocols are for reference only. Solarbio does not independently validate these methods. 温馨提示： 使用前请离心。如有问题请及时联系我们。 Quick spin before use. If you have any questions, please contact us. 实验图  Western blot analysis with Symmetric DiMethyl-Histone H3-R2 antibody diluted at 1:1000 |
Anti-Symmetric DiMethyl-Histone H3-R2 Polyclonal Antibody | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 宿主 | Rabbit | ||||||||||||||||||||||||||||||||||||||||||
| 别名 | H3.4;H3/g;H3FT;H3t | ||||||||||||||||||||||||||||||||||||||||||
| 稀释比例 | WB 1:500-2000. IHC 1:50-200. IF 1:50-200. IP 1:50-200. ChIP 1:20-100. ChIPseq 1:20-100. | ||||||||||||||||||||||||||||||||||||||||||
| 蛋白分子量 | 16kDa | ||||||||||||||||||||||||||||||||||||||||||
| Gene ID | 8290 | ||||||||||||||||||||||||||||||||||||||||||
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. | ||||||||||||||||||||||||||||||||||||||||||
| 储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | ||||||||||||||||||||||||||||||||||||||||||
| 纯化方法 | Affinity purification | ||||||||||||||||||||||||||||||||||||||||||
| 亚型 | IgG | ||||||||||||||||||||||||||||||||||||||||||
| 免疫原 | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 | ||||||||||||||||||||||||||||||||||||||||||
| 性状 | 液体 | ||||||||||||||||||||||||||||||||||||||||||
| Public Immunogen Range | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 | ||||||||||||||||||||||||||||||||||||||||||
| 亚细胞定位 | Chromosome Nucleus | ||||||||||||||||||||||||||||||||||||||||||
| Swiss Prot | Q16695 | ||||||||||||||||||||||||||||||||||||||||||
| 克隆类型 | Polyclonal Antibody | ||||||||||||||||||||||||||||||||||||||||||
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. | ||||||||||||||||||||||||||||||||||||||||||
| 应用 | WB IHC IF IP ChIP ChIPseq | ||||||||||||||||||||||||||||||||||||||||||
| 交叉反应 | Human Mouse Rat | ||||||||||||||||||||||||||||||||||||||||||
| 规格 | 50ul 100ul |
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
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