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Human SAA4 Protein (GST Tag)

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  • ¥2159.15
  • Frdbio
  • WUHAN,CHINA
  • FrdH2535-909E
  • 2025年11月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      50μg

    分子别名(Synonym):C-SAA,CSAA,SAA4 种属(Species):Human 表达系统(Expressed Host):E. coli 制剂(Buffer):Lyophilized from sterile PBS, 0.5% Triton X-100, pH 7.4 分子量(Molecule Mass):The recombinant human SAA4 /GST chimera consists of 344 amino acids and has a predicted molecular mass of 39.8KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions. 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity): 表达区间(Protein Construction):A DNA sequence encoding the mature form of human SAA4 (P35542) (Glu19-Tyr130) was fused with the GST tag at the N-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    图标文献和实验
    相关实验
    • GST Fusion Protein Prep

      Thaw out 1 ml aliquot of fusion protein lysate. Centrifuge to remove precipitate. Incubate 1 ml lysate with 0.4 ml 50% GST beads. Incubate at 4C with mixing for 1 hour. Centrifuge 5K 2' in 1.5 ml eppendorf tubes. Wash the beads 4X with PBST

    • GST Fusion Protein Preparation

      CLEAVAGEAlternate Mini PrepThaw out 1 ml aliquot of fusion protein lysate.Centrifuge to remove precipitate.Incubate 1 ml lysate with 0.4 ml 50% GST beads.Incubate at 4℃ with mixing for 1 hour.Centrifuge 5K 2' in 1.5 ml eppendorf tubes.Wash the beads 4X with PBST

    • Thrombin Cleavage of GST-Fusion protein

      . Keep each supernatant aside 4. As a control you can elute the remaining uncleaved protein (still attached to the resin through the GST) by extraction several times with elution buffer. 5. Check results by PAGE-SDS gels or immediately separate the cleavage products

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