Mouse BACE-1 Protein (His Tag)

分子别名（Synonym）:C76936 种属（Species）：Mouse 表达系统（Expressed Host）：HEK293 Cells 制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4 分子量（Molecule Mass）：The secreted recombinant mouse BACE1 consists of 447 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions. 内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method. 生物活性(Activity)：Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004, RCat:D Systems). Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. 表达区间（Protein Construction）：A DNA sequence encoding the extracellular domain (Ser 2-Thr 457) of mouse BACE1 (NM_011792.5) precursor was expressed with a C-terminal polyhistidine tag. 存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.