Human CD3E & CD3G Heterodimer

分子别名（Synonym）:CD3E & CD3G Heterodimer,IMD18,T3E,TCRE 种属（Species）：Human 表达系统（Expressed Host）：HEK293 Cells 制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4. 分子量（Molecule Mass）：The recombinant heterodimer of human CD3E/CD3G comprises 683 (355+328) amino acids and has a calculated molecular mass of 77.2 (40.1+37.1) KDa. As a result of glycosylation, the apparent molecular mass of human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions. 内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method. 生物活性(Activity)：Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. 表达区间（Protein Construction）：A DNA sequence encoding the extracellular domain (Ser2-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Ser2-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. 存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.