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Human RNase A / Ribonuclease A

/ RNASE1 Protein (His Tag)
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  • ¥1671.60
  • Frdbio
  • WUHAN,CHINA
  • FrdH4692-802H
  • 2025年10月05日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      20μg

    分子别名(Synonym):RAC1,RIB1,RNS1 种属(Species):Human 表达系统(Expressed Host):HEK293 Cells 制剂(Buffer):Lyophilized from sterile PBS, pH 7.4 分子量(Molecule Mass):The recombinant human RNASE1 comprises 139 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 29 ,26 and 22 kDa in SDS-PAGE under reducing conditions 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity):Measured by its ability to hydrolyze yeast RNA(Sigma,Catalog # R9001). One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37℃ and pH 7.0. The specific activity is >2.5×106 unit/mg. 表达区间(Protein Construction):A DNA sequence encoding the human RNASE1 (P07998) (Ser2-Thr156) was expressed with a polyhistidine tag at the C-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    图标文献和实验
    相关实验
    • The Basics: RNase Control

      The traditional method for combating RNases in enzymatic reactions such as in vitro transcription, reverse transcription and translation is to use human placental ribonuclease inhibitor (also known as RNase Inhibitor Protein, RI or hPRI

    • Human FastTrack mRNA Isolation

      . Modified FastTrack 2.0 Protocol Isolation of mRNA 1.Add 15ml of Lysis Buffer (15ml Stock Buffer + 300 ul RNase/Protein Degrader) to the frozen pellet. 2.The tissue homogenizer should be cleaned so that it is RNase-free. At a minimum will require

    • Identifying Chromosomal Targets of DNA‐Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE)

      .3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding

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