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- 文献和实验
- 技术资料
- 保存条件：
保存在2-8℃，6个月。
- 保质期：
6个月
- 英文名：
Soil Dehydrogenase (S-DHA) Activity Assay Kit
- 库存：
99
- 供应商：
北京索莱宝科技有限公司
- CAS号：
BC0395-100T/48S
- 规格：
100管/48样
需自备的仪器和用品：
可见分光光度计/酶标仪、微量玻璃比色皿/96孔板（非聚苯乙烯材质）、研钵、30-50目筛、天平、恒温培养箱/水浴锅、低温离心机、冰、蒸馏水、乙酸乙酯（不允许快递）。
操作步骤：具体操作步骤详见“产品资料”附件
注意事项：
- 试剂三易挥发，有毒，为了您的健康，请穿实验服，戴口罩，戴乳胶手套操作。
- 如果测定出来的吸光值较大，减少样本用量再进行测定；若吸光值过小则延长培养时间。注意同步修改计算公式。
- 如果离心后待测的上清依然浑浊，可尝试加大离心转速或延长时间，例如15000rpm，4℃，离心20min。
- Hou Q, Wang W, Yang Y, et al. Rhizosphere microbial diversity and community dynamics during potato cultivation[J]. European Journal of Soil Biology, 2020, 98: 103176.
- Kumar S, Chaudhuri S, Maiti S K. Soil dehydrogenase enzyme activity in natural and mine soil-a review[J]. Middle-East Journal of Scientific Research, 2013, 13(7): 898-906.
- Friedel J K, Mölter K, Fischer W R. Comparison and improvement of methods for determining soil dehydrogenase activity by using triphenyltetrazolium chloride and iodonitrotetrazolium chloride[J]. Biology and fertility of soils, 1994, 18(4): 291-296.
若使用96孔板测定，不可使用聚苯乙烯材质，建议使用96孔石英板
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