LS-F2523 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat NGF in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of NGF as low as 1 picograms per milliliter.
This kit is for the detection of natural and recombinant rat NGF. No detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.
Manual
Intended Sample Types
Cell Culture Supernatants, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.6 - 1000 pg/ml
Sensitivity
1 pg/ml
Precision
Intra-Assay: CV%<6% Inter-Assay: CV%<7.2%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components