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- 详细信息
- 文献和实验
- 技术资料
- 库存:
128
- 英文名:
Recombinant Human Interferon-stimulated Gene 15
- 保质期:
6个月
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
收到货后请置于-20℃
- 规格:
10μg|50μg|500μg|1mg
特别提示:包括重组人泛素样蛋白(ISG15)在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人泛素样蛋白(ISG15)
英文名称:Recombinant Human Interferon-stimulated Gene 15
产品货号:JN0883
产品规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Gly2-Gly157在C端含有His标签。
ISG15质量控制:>95%(还原性SDS-PAGE)
ISG15制剂:液体
ISG15保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于ISG15:
Ubiquitin-Like Protein ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge.The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation.ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.
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文献和实验qiyichen 请高手不吝赐教 qiyichen 泛素属于胞内、胞浆还是胞核蛋白? sosorunning Subcellular location: Cytoplasm and Nucleus. windcmt 用于蛋白质降解的,应该主要在胞浆,细胞核内也有。 蛋白质降解途径之2---以来泛素和ATP的途径 本文由丁香园论坛
植物源重组人胰岛素生长因子融合蛋白(OsrIGF-1),胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白,分子量7649Da,耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用,它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白(OsrIGF-1)在IGF-1的N末端融合了人血清白蛋白(HSA),HSA作为蛋白质保护剂增加了IGF
0:10 内容简介10 0:21 订阅内容21 0:42 目的蛋白的表达和纯化、泛素标记42 3:15 免疫沉淀反应195 4:51 SDS-PAGE电泳和免疫染色291 5:48 体外检测蛋白质泛素化348 7:02 代表性结果422 8:22 结论502 蛋白质泛素化检测本视频来源于网络,如有异议请联系我们,我们将在5个工作日内作出处理。
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