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Mouse Anti Human Ras associati

on domain-containing protein 1
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  • ¥1080 - 7500
  • Prospecbio
  • 以色列
  • ant-400
  • 2025年07月18日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      RASSF1A

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/100ug

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:100ug产品价格:¥7500.0

    产品细节图片1

    CATALOGUE NUMBER

    ANT-400

    SYNONYMS

    Ras association domain-containing protein 1, 123F2, RDA32, NORE2A, RASSF1A, REH3P21, RASSF1.

    TYPE

    Mouse Anti Human Monoclonal.

    INTRODUCTION

    RASSF1A is a protein similar to the RAS effector proteins. Loss or altered expression of the RASSF1A gene has been linked to the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of the RASSF1A gene. The inactivation of the RASSF1A gene was found to be interconnected with the hypermethylation of its CpG-island promoter region. The RASSF1A protein was found to interact with DNA repair protein XPA. RASSF1A was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest.

    CLONE

    P3F3AT.

    IMMUNOGEN

    Anti-human RASSF1A mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human RASSF1A amino acids 1-340 purified from E. coli.

    IG SUBCLASS

    Mouse IgG1 heavy chain and κ light chain.

    PURIFICATION METHOD

    RASSF1A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

    PHYSICAL APPEARANCE

    Sterile Filtered clear solution.

    FORMULATION

    1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

    STORAGE PROCEDURES

    For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

    STABILITY / SHELF LIFE

    12 months at -20°C. 1 month at 4°C.

    APPLICATIONS

    RASSF1A antibody has been tested by ELISA, Western blot and immunocytochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2,000. Recommended starting dilution is 1:1,000.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    RASSF1A is a protein similar to the RAS effector proteins. Loss or altered expression of the RASSF1A gene has been linked to the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of the RASSF1A gene. The inactivation of the RASSF1A gene was found to be interconnected with the hypermethylation of its CpG-island promoter region. The RASSF1A protein was found to interact with DNA repair protein XPA. RASSF1A was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest.

    相关实验
    • Anti-HA Immunocytology

        The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein

    • A Novel Method for the Production of Fully Modified K-Ras 4B

      post-translationally modified proteins in bacterial expression systems is often problematic. Here, we describe a novel recombinant approach to prepare human K-Ras 4B, a protein that is post-translationally farnesylated

    • Anti-HA Immunocytology

        Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots

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    文献支持
    Mouse Anti Human Ras association domain-containing protein 1
    ¥1080 - 7500