Recombinant Ja.pan.ese en.ceph

Japanese encephalitis previously known as Japanese B encephalitis is a virus from the family Flaviviridae. it is closely related to the We.st Ni.le vir.us and St. Louis encephalitis virus. Positive sense single stranded RNA genome is packaged in the capsid, formed by the capsid protein. The outer envelope is formed by envelope (E) protein and is the protective antigen. It aids in entry of the virus to the inside of the cell. The genome also encodes several nonstructural proteins also (NS1,NS2a,NS2b,NS3,N4a,NS4b,NS5). NS1 is produced as secretory form also. NS3 is a putative helicase, and NS5 is the viral polymerase. It has been noted that the Ja.pan.ese en.ceph.alitis vi.rus (JEV) infects the lumen of the endoplasmic reticulum (ER) and rapidly accumulates substantial amounts of viral proteins for the JEV. Japanese Encephalitis is diagnosed by detection of antibodies in serum and CSF (cerebrospinal fluid) by IgM capture ELISA.

The E.coli derived recombinant protein contains full length Ja.pan.ese en.ceph.alitis vi.rus ENV antigen having an Mw of 50kDa. The protein is fused to a 6 histidines tag. GenBank: AHK05344.1

Escherichia Coli.

Encephalitis protein was Purified by proprietary chromatographic technique.

Encephalitis protein is >90% pure as determined by SDS-PAGE (coomassie staining).

20mM Tris-MES pH 6.5, 8M urea, 200mM NaCl and 0.05% Tween-20.

Encephalitis protein although stable at 4°C for 1 week, should be stored below -18°C. 
Please prevent freeze thaw cycles.

SDS

Immunoreactive with sera from JEV-infected individuals.

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