- ￥1080 - 87906
- Prospecbio
- 以色列
- pro-1609
- 2026年01月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
WFDC2, His
- 库存：
常规产品有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/1mg
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 1mg | 产品价格： | ￥87906.0 |
CATALOGUE NUMBER
PRO-1609
SYNONYMS
INTRODUCTION
DESCRIPTION
SOURCE
PHYSICAL APPEARANCE
FORMULATION
SOLUBILITY
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. WFDC2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
STABILITY
PURITY
AMINO ACID SEQUENCE
SAFETY DATA SHEET
SDS
USAGE
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文献和实验WFDC2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (31-124) containing 104 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 11.3kDa (calculated).
Strategies to Optimize Protein Expression in E. coli
., Wang, Y., Ma, J.B., Gao, D.M., Wu, H.M., and Chen, H.B. 2006. Over‐expression and purification of isotopically labeled recombinant ligand‐binding domain of orphan nuclear receptor human B1‐binding factor/human liver receptor homologue 1 for NMR studies
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
) was exchanged with the multiple cloning site of pET15b (sequences between the Xba I to Bpu 1102I sites). The HMK coding sequence was then inserted by recombinant PCR directly downstream of and in frame with the His tag
的吸附在固相介质上，例如离子交换介质、金属螯合介质等，可以避免伸展的多肽分子之间形成聚集体。目标蛋白上的His-tag [8] 和Cellulose-binding domain[9]在伸展的状态下仍旧保持结合的能力。 最近层析复性方法成为研究的热点，不仅可以降低聚集体的形成，还可以适用于大规模生产，同时对目标蛋白质有纯化作用。根据复性方式的不同，可以把层析复性方法分为三种[10]： 1) 基于缓冲液置换机理的凝胶过滤层析 2) 蛋白质可逆的直接吸附在介质上，随后除去变性剂诱导
技术资料