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Recombinant Human RAD51B

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  • ¥1080 - 45885
  • Prospecbio
  • 以色列
  • pro-1804
  • 2025年07月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      RAD51B

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      5ug/20ug/1mg

    规格:5ug产品价格:¥1080.0
    规格:20ug产品价格:¥2415.0
    规格:1mg产品价格:¥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-1804

    SYNONYMS

    RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B.

    INTRODUCTION

    RAD51 family members are evolutionarily conserved proteins vital for DNA repair by homologous recombination. RAD51B is a part of the RAD51 protein family. RAD51B is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks occurring during DNA replication or induced by DNA-damaging agents. RAD51B forms a stable heterodimer with the family member RAD51C, which interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of RAD51B leads to cell cycle G1 delay and cell apoptosis.

    DESCRIPTION

    RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered clear solution.

    FORMULATION

    The RAD51B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 85% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    MG.S.SHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS G.S.SCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS.

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    相关实验
    • Assay for Human Rad51-Mediated DNA Displacement Loop Formation

      as described by  Chi et al. (2007) . Purify Rad54 as described by  Sigurdsson et al. (2002) . Recombinant human Rad51, purified (5-10 µM) Purify as described by  Sigurdsson et al. (2001) .   SDS (Sodium dodecyl sulfate; 

    • Biochemical Studies on Human Rad51-Mediated Homologous Recombination

      factors. The development of various biochemical analyses with the requisite purified factors provides an opportunity to understand the molecular mechanisms of HR. In this chapter, we describe detailed procedures of in vitro assays using human Rad51

    • Construction of Recombinant Human Cytomegalovirus

      The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral

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    文献支持
    Recombinant Human RAD51B
    ¥1080 - 45885