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Recombinant Protein L

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  • ￥1080 - 16000
  • Prospecbio
  • 以色列
  • pro-1790
  • 2026年01月22日
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    上海经科化学科技有限公司
    16 年钻石会员

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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      Protein-L

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      1mg/10mg/100mg

    规格：1mg产品价格：￥1080.0
    规格：10mg产品价格：￥2415.0
    规格：100mg产品价格：￥16000.0

    产品细节图片1

    CATALOGUE NUMBER

    PRO-1790

    SYNONYMS

    SPL.

    INTRODUCTION

    The recombinant Protein L is genetically engineered protein and holds 5 IgG-binding regions of protein L and it has the exclusive capability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding. The recombinant Protein L is perfect for purification of polyclonal or monoclonal IgG antibodies and it binds to human, mouse, rat and pig IgG.

    DESCRIPTION

    Protein-L Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 365 amino acids and having a molecular mass of 40.5 kDa but it migrates with an apparent molecular mass of 45 kDa in SDS-PAGE.
    The Protein-L is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Protein-L was lyophilized with no additives.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Protein-L in sterile water or saline not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Protein-L although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein-L should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 97.0% as determined by:
    (a) Analysis by SEC-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE
    EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG

    SAFETY DATA SHEET

    SDS

    USAGE

    Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献
    Protein-L Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 365 amino acids and having a molecular mass of 40.5 kDa but it migrates with an apparent molecular mass of 45 kDa in SDS-PAGE.
    The Protein-L is purified by proprietary chromatographic techniques.
    相关实验

    • Purification of recombinant sBRF M166L

      culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g

    • Purification of recombinant sBRF M166L

      with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell

    • Recombinant Protein Production in Yeasts

      Recombinant protein production is a multibillion-dollar market. The development of a new product begins with the choice of a production host. While one single perfect host for every protein does not exist, several expression systems ranging

    查看更多相关实验 >

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    Recombinant Protein L
    ￥1080 - 16000