产品封面图
文献支持

Recombinant E.Coli Pyruvate Ki

nase I
收藏
  • ￥1080 - 45885
  • Prospecbio
  • 以色列
  • pka-028
  • 2025年07月11日
    avatar
    上海经科化学科技有限公司
    16 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      pykF

    • 库存：

      常规产品有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      5ug/20ug/1mg

    规格：5ug产品价格：￥1080.0
    规格：20ug产品价格：￥2415.0
    规格：1mg产品价格：￥45885.0

    产品细节图片1

    CATALOGUE NUMBER

    PKA-028

    SYNONYMS

    Pyruvate kinase I, PK-1, pykF, b1676, JW1666.

    INTRODUCTION

    pykF (pyruvate kinase I) is a member of the pyruvate kinase family. pykF is an enzyme involved in glycolysis. pykF catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP, a process which also requires a Magnesium ion. This is the final step in the glycolytic pathway, which produces pyruvate molecules, the ultimate product of aerobic glycolysis.

    DESCRIPTION

    pykF E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 494 amino acids (1-470 a.a) and having a molecular mass of 53.3kDa.
    pykF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    SOURCE

    E.coli.

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    pykF protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 95% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    MG./S-SHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献

    pykF E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 494 amino acids (1-470 a.a) and having a molecular mass of 53.3kDa.
    pykF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    相关实验

    • A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

      in transglycosylation reaction. In the assay, UDP was coupled to the conversion of phosphoenolpyruvate to pyruvate using pyruvate kinase. Using a commercial pyruvate assay kit, the pyruvate was converted to a red terminal product, which could be photometrically measured

    • 细胞器DNA文库构建

      final volume (final DNA concentration = 5 ng/ul) Incubate o.n. at 14'C.VII. TRANSFORMATION In E.coli XL1. Expect 20 recombinant clones per ng of insert DNA. DNA Nebulization (Okpodu CM, Robertson D, Boss WF, Togasaki RK, Surzycki SJ (1994

    • Organelle DNA Library Construction

      In E.coli XL1. Expect 20 recombinant clones per ng of insert DNA. DNA Nebulization (Okpodu CM, Robertson D, Boss WF, Togasaki RK, Surzycki SJ (1994). Biotechniques 16, 154-159.) 1. Device a.) E.g., BioNeb Cell Disruption System, from Glas

    查看更多相关实验 >

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Recombinant E.Coli Acetate Kinase
    ￥1080
    上海经科化学科技有限公司
    2025年07月13日询价
    PYRUVATE KINASE
    询价
    西宝生物科技(上海)股份有限公司
    2025年07月15日询价
    Recombinant Escherichia coli Pyruvate kinase I (pykF)
    询价
    默瑞（上海）生物科技有限公司
    2025年07月12日询价
    Recombinant Human Tumor Type M2 Pyruvate Kinase
    ￥987
    上海优宁维生物科技股份有限公司
    2025年08月28日询价
    Recombinant Human Pyruvate kinase PKM/PKM2
    ￥5760
    上海吉至生化科技有限公司
    2025年07月15日询价
    文献支持
    Recombinant E.Coli Pyruvate Kinase I
    ￥1080 - 45885