Recombinant Rat Carboxypeptida

Carboxypeptidase B, Cpb1, Cpb.

Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kDa, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.

Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques.
Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.

Escherichia Coli.

Sterile Filtered lyophilized powder.

The protein was lyophilized with 100mM NaCl, mannitol and 20mM Tris pH-7.5.

It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18MΩ-cm H2O or 25mM Tris-HCl pH 7.65 not less than 100µg/ml , which can then be further diluted to other aqueous solutions.

Store the lyophilized Carboxypeptidase-B at 4°C. Upon reconstitute the protein should be stored at 4°C for 2 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.

Greater than 90% as determined by SDS-PAGE.

One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

170 units/mg protein.

SDS

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