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Recombinant Human Adenosylmeth

ionine Decarboxylase 1
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  • ¥1080 - 20286
  • Prospecbio
  • 以色列
  • enz-624
  • 2025年12月31日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      AMD1

    • 库存

      常规产品有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      1ug/5ug/50ug

    规格:1ug产品价格:¥1080.0
    规格:5ug产品价格:¥2415.0
    规格:50ug产品价格:¥20286.0

     

    CATALOGUE NUMBER

    ENZ-624

    SYNONYMS

    Adenosylmethionine decarboxylase 1, S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, S-adenosylmethionine decarboxylase 1, SAMDC, AMD, EC 4.1.1.50.

    INTRODUCTION

    Adenosylmethionine decarboxylase proenzyme (AMD1) is synthesized originally as an inactive proenzyme. Putrescine stimulates both the proenzyme processing and the catalytic activity. The catalytic activity is inhibited by iodoacetic acid. The active enzyme formation entails a self-maturation process in which the active site pyruvoyl group is produced from an internal serine residue using an autocatalytic post-translational modification.

    DESCRIPTION

    AMD1 Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (68-334) and having a molecular mass of 33.4 kDa.
    AMD1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    SOURCE

    E.coli.

    PHYSICAL APPEARANCE

    Sterile Filtered colorless solution.

    FORMULATION

    The AMD1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.

    STABILITY

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Avoid multiple freeze-thaw cycles.

    PURITY

    Greater than 80% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    G.-S/SHHHHHH SSGLVPRGSH MGSHMSSMFV SKRRFILKTC GTTLLLKALV PLLKLARDYS GFDSIQSFFY SRKNFMKPSH QGYPHRNFQE EIEFLNAIFP NGAAYCMGRM NSDCWYLYTL DFPESRVISQ PDQTLEILMS ELDPAVMDQF YMKDGVTAKD VTRESGIRDL IPGSVIDATM FNPCGYSMNG MKSDGTYWTI HITPEPEFSY VSFETNLSQT SYDDLIRKVV EVFKPGKFVT TLFVNQSSKC RTVLASPQKI EGFKRLDCQS AMFNDYNFVF TSFAKKQQQQ QS

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
     

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    图标文献和实验
    该产品被引用文献

    AMD1 Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (68-334) and having a molecular mass of 33.4 kDa.
    AMD1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    相关实验
    • 免疫技术

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    • Studies of the Ubiquitin Proteasome System

      ( protocol 34 ) E2‐UbcH5B ( protocol 37 ) GST‐ubiquitin ( protocol 29 ) Human APP‐BP1/Uba3 (NEDD8 E1; protocol 21 ) Ubc12 (E2

    • Polony DNA Sequencing

      ) 1.6 mM S ‐adenosylmethionine (SAM; diluted from 32 mM stock in 1× NEBuffer 4; NEB) 2 U/µl Mme I (NEB)

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    文献支持
    Recombinant Human Adenosylmethionine Decarboxylase 1
    ¥1080 - 20286