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- cyt-1015
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
for future use below -18°C
- 保质期：
See instructions
- 英文名：
MCSF, Sf9 His
- 库存：
部分小规格有备货
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 规格：
2ug/10ug/100ug
| 规格： | 2ug | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 10ug | 产品价格： | ￥2415.0 |
| 规格： | 100ug | 产品价格： | ￥20286.0 |
CATALOGUE NUMBER
CYT-1015
SYNONYMS
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim.
INTRODUCTION
Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. MCSF induces cells of the monocyte/macrophage lineage. MCSF plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
DESCRIPTION
MCSF produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 231 amino acids (33-255 a.a.) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
MCSF is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
SOURCE
Sf9, Insect cells.
PHYSICAL APPEARANCE
Sterile filtered colorless solution.
FORMULATION
MCSF protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by SDS-PAGE.
BIOLOGICAL ACTIVITY
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cell. The ED50 for this effect is less or equal to 3 ng/ml.
AMINO ACID SEQUENCE
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPRLEHHHHH H.
SAFETY DATA SHEET
SDS
USAGE
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文献和实验MCSF produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 231 amino acids (33-255 a.a.) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
MCSF is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Acute myeloid leukemia (AML) has an annual incidence of 2.4 cases per 100,000 persons and is the most common form of acute leukemia in adults (1 , 2 ). AML is a model for drug-resistant human cancers, and current therapies for AML possess
Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms
With the completion of the first map of the entire human genome, it is estimated that there are approx 35,000 genes, which encode for translated RNA and protein products (1 ,2 ). The difference between any two human genomes is estimated
The hematopoietic growth factor granulocyte colony-stimulating factor (G-CSF) has been in the clinic since 1986, when the clinical development of bacterially synthesized recombinant filgrastim began (1 ). A glycosylated form of G-CSF
技术资料