LS-F24614 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MIF in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MIF as low as 20 picograms per milliliter.
For the detection and quantification of endogenous levels of natural and/or recombinant Human MIF proteins.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
156 - 10000 pg/ml
Sensitivity
20 pg/ml
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene. This gene is located to human chromosome 22q11.2. It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.
Restrictions