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Full length human Cyclin D1 Recombinant protein.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Rat, Cat, Dog
12 months from the shipping date of the product.
Human
GTX108624
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA
1.21 mg/ml (Please refer to the vial label for the specific concentration.)
IP/MS validation was supported by references (PMID:30377392)
Cyclin D1 antibody [N1C3]
Cyclin D1 抗体 [N1C3]
100 μl/25 μl
规格: | 100 μl | 产品价格: | ¥4000.0 |
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规格: | 25 μl | 产品价格: | ¥1700.0 |
Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 μg of Cyclin D1 antibody [N1C3] (GTX108624).
Western blot analysis was performed using Cyclin D1 antibody [N1C3] (GTX108624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (GTX108624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Cyclin D1 stained by Cyclin D1 antibody (GTX108624) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sandwich ELISA detection of recombinant full-length Cyclin D1 protein using GTX634347 as capture antibody at concentration of 5 μg/mL and GTX108624 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Cyclin D1 antibody [N1C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% PFA at RT for 15 min.
Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] (GTX108624) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Scale bar = 10 μm.
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