- ￥1700 - 4000
- GeneTex
- 美国
- GTX108298
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Wm
- Rabbit
- Human, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2A.Z. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX108298
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Wm
- 浓度：
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2A.Z
- 抗体英文名：
Histone H2A.Z antibody
- 抗体名：
Histone H2A.Z 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Immunohistochemical analysis (whole mount) of zebrafish embryo, using Histone H2A.Z antibody (GTX108298) at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: JurKat
15% SDS PAGE
GTX108298 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using Histone H2A.Z(GTX108298) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed A431, using Histone H2A.Z(GTX108298) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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文献和实验Piquet S et al., Mol Cell 2018 (PMID:30344095)
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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