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Histone H2A.X antibody

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • 2025年07月15日
  • WB, ICC/IF, IHC-P, IP
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX108272

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IP

    • 浓度

      0.81 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      Histone H2A.X

    • 抗体英文名

      Histone H2A.X antibody

    • 抗体名

      Histone H2A.X 抗体

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis.
    Sample: Paraffin-embedded human esophagus cancer.
    Histone H2A.X antibody (GTX108272) diluted at 1:500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片2

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis.
    Sample: Paraffin-embedded human cervical cancer.
    Histone H2A.X antibody (GTX108272) diluted at 1:500.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片3

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse prostate.
    Histone H2A.X antibody (GTX108272) dilution: 1:500.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片4

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis.
    Sample: Paraffin-embedded rat hind brain.
    Histone H2A.X antibody (GTX108272) dilution: 1:500.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片5

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse prostate.
    Histone H2A.X antibody (GTX108272) dilution: 1:500.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    产品细节图片6

    Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

    产品细节图片7

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis.
    Sample: Paraffin-embedded human breast carcinoma.
    Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

    产品细节图片8

    Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.
    IP samples: Jurkat whole cell extract
    A. 40 μg Jurkat whole cell extract
    B. Control with 4 μg of preimmune Rabbit IgG
    C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272)
    15 % SDS-PAGE
    The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
    [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

    产品细节图片9

    Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
    A. 50 μg rat brain extract
    12% SDS-PAGE
    Histone H2A.X antibody (GTX108272) dilution: 1:1000
    The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

    产品细节图片10

    Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis.
    Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
    Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
    Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
    Scale bar= 10 μm.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Liu X et al., Mol Cancer Ther 2019 (PMID:31501277)
    Lee CY et al., Nat Commun 2019 (PMID:30622262)
    Yu CY et al., Eur J Pharmacol 2017 (PMID:28986085)
    Peraldo-Neia C et al., BMC Cancer 2014 (PMID:25479910)
    Chinnam M et al., J Natl Cancer Inst 2014 (PMID:25296641)
    Liu MK et al., Int J Mol Sci 2016 (PMID:27314325)
    Yuan SS et al., J Natl Cancer Inst 2012 (PMID:22914783)
    Chen HM et al., Free Radic Biol Med 2011 (PMID:21256211)
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    • HISTONE KINASE ASSAY

        PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined

    图标技术资料

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    文献支持
    Histone H2A.X antibody
    ¥1700 - 4000