CFSE / 7-AAD双染细胞毒性检测试剂盒

Fluorescein labeling of proteins

This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable

Fluorescein labeling of proteins

Fluorescein labeling of proteins This method uses carboxyfluorescein succinimidyl ester (CFSE) rather than fluorescein isothiocyanate, resulting

体外微核试验原理及注意事项

淋巴瘤细胞株)，建议采用方案二。2.4 剂量2.4.1 剂量设置至少应设置 3 个检测剂量。受试物没有细胞毒性时，从最高剂量往下设至少 2 个剂量，一般情况下间隔系数可为 2～3；有细胞毒性时，其剂量范围应涵盖从 55% 士 5% 的细胞毒性到几乎无细胞毒性。2.4.2 最高剂量的选择决定最高剂量的因素是细胞毒性、受试物的溶解度以及 pH、渗透压。受试物有细胞毒性时，最高剂量应能引起 55% 士 5% 的细胞毒性；如果没有细胞毒性或沉淀，最高剂量应是 5 μL/mL、5 mg/ml 或 10 mmol/L。对溶解