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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
PI3-Kinase p150 antibody [N1N2], N-term
- 抗体名:
PI3-Kinase p150 antibody [N1N2], N-term
- 规格:
100μl
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文献和实验Immunoprecipitation and Immune Complex kinase assay
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) In the cold room, wash cells with cold "Tris" (the same stuff used for TC). Spin down the cells if you are working with suspension cells in a low speed
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
and resuspending. 10)If doing kinase assay,do additional wash in TN and then proceed with kinase assay (below). 11)Pellets can be stored dry at -20℃ for short term (one or two days)or at-70℃ for longer. 12)Resuspend pellet in at least 50 microliters sample
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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